Coupling of T161 and T14 phosphorylations protects cyclin B–CDK1 from premature activation

摘要

Whether CDK1 phosphorylations occur on the same molecules could be determined using two-dimensional gel electrophoresis. The activating T161 phosphorylation was found to be tightly coupled to the T14 inhibitory phosphorylation in cyclin B1–CDK1, suggesting that the mitotic timer is endowed with an intrinsic mechanism protecting it from premature activation by CAK.